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KMID : 0380020060210010016
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Korean Journal of Biotechnology and Bioengineering
2006 Volume.21 No. 1 p.16 ~ p.19
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Two-dimensional Electrophoretic Analysis of Nucleotide Phosphate Kinase Mediated Hydrogen Peroxide Cross-Linking in Saccharamyces cerevisiae
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Moon Hae-Jeong
Yun Dae-Jin
Park Chang-Ho
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Abstract
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Oxidative modification of nucleoside diphosphate kinase (NDPK) is identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometer. The quaternary structure of NDPK appears to be regulated by cross-linking with an oxidant, . We compared roles of NDPK in each of wild type and ynk mutant against oxidative stress. Six specific proteins changed by were identified using two-dimensional electrophoretic analysis. YNK regulated several proteins, related to signaling functions. These results suggest that one of the important functions of NDPK is the regulation of cellular redox state.
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KEYWORD
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NDPK, two-dimensional electrophoretic analysis, ynk mutant, MALDI-TOF MS
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